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51.
不同脑缺血和再灌流过程中大鼠脑组织NO含量的动态变化 总被引:12,自引:0,他引:12
采用线栓法制成大鼠大脑中动脉梗塞 ( MCAO)模型 ,依 Hb O2 - NO法测定持续性脑缺血和缺血 /再灌流脑组织内 NO含量的变化 ,以探讨不同脑缺血和再灌流过程中 NO的变化规律及其意义。结果 :缺血 3小时受损脑组织 NO水平即增高 ,再灌流后 NO逐步升高 ,而持续性缺血状态下 NO则表现降低后再升高的变化。虽然两组 NO在 7天时均有明显降低 ,但仍高于缺血前水平。认为持续性脑缺血和缺血 /再灌流情况下 NO的变化规律有所不同 ,与缺血脑组织的缺氧及产生 NO所需底物供应缺乏有关 ,且可能与脑组织的损害密切相关 相似文献
52.
为证明癫痫发作早期一氧化氮(NO)抗发作效应,用NO合酶(NOS)抑制剂L-硝基精氨酸甲酯(L-NAME)对大鼠红藻氨酸(KA)诱导性发作进行干预,同时用分光光度法检测海马结构中NOS活性的早期变化。发现KA发作10min、30min组海马结构中NOS活性明显升高,而KA注射前30min给予L-NAME可显著抑制NOS活性的升高,这种抑制效应与大鼠KA发作中湿狗样摇动(WDS)的提早出现和发生次数增多显著相关。结果提示在KA诱导大鼠发作早期内源性NO具有明显的抗发作效用。 相似文献
53.
紧闭式氧化亚氮麻醉方法的探讨 总被引:2,自引:1,他引:1
25例选择期手术病人采用紧闭式氧化亚氮麻醉方法,术中持续监测呼气末氧和氧化亚氮浓度,脉搏血氧饱和度和呼吸循环指标,术中观察紧闭式麻醉后呼吸末氧化亚氮,氧浓度变化,结果:紧闭式麻醉1,2,3h后氧化亚氮浓度分别为52.7%,56%,64.9%,氧浓度为42.1%,34.4%,30.8%,随麻醉时间的延长,气道压力先降后回升,约3h恢复至紧闭麻醉前的水平,紧闭式麻醉前后在本组观察时间内动脉血气分析提示 相似文献
54.
Ilham Bettahi David Pozo Carmen Osuna Russel J. Reiter Dario Acuña-Castroviejo Juan M. Guerrero 《Journal of pineal research》1996,20(4):205-210
Abstract: In this report, rat hypothalamic nitric oxide synthase (NOS) activity is shown to be partially inhibited by physiological concentrations of the pineal hormone melatonin. In vitro studies demonstrate that 1 nM melatonin, which approximates the physiological concentration of the hormone at night, significantly inhibited NOS activity. In vivo studies show that administering melatonin or collecting the hypothalamus from animals at night, when endogenous melatonin levels are elevated, results in a significant decrease of NOS activity. Results also show that calmodulin may be involved in this process since its presence in the incubation medium prevents the inhibitory effect of melatonin on NOS activity. 相似文献
55.
Abstract Nitric oxide (NO) plays an important physiological role in regulating gastrointestinal motility. Involvement of endogenous NO was evaluated in the response to non-adrenergic, non-cholinergic (NANC) nerve stimulation of the dog sphincter muscle of Oddi. Transmural electrical stimulation (TES), nicotine (10?5M) and K+ (10 mM) produced only a relaxation in the sphincter muscle strips contracted with substance P, which was not potentiated by atropine. The TES-induced relaxation was abolished by tetrodotoxin (3 times 10?7 M) and oxyhaemoglobin (1.6 times 10?5 M), but not affected by atropine (10?7 M), propranolol (10?7 M), phentolamine (10?7 M), indomethacin (10?6 M), chole-cystokinin (CCK, 10?8 M) and vasoactive intestinal polypeptide (VIP, 10?8 M). The relaxation was also abolished by treatment with NG-nitro-L-arginine (L-NA, 10?5 M), an NO synthase inhibitor. Nicotine produced a transient relaxation, which was abolished by tetrodotoxin, hexamethonium (10?5 M) and L-NA, but not affected by atropine and NG-nitro-D-arginine (D-NA, 10?5 M). The addition of K+ elicited a transient relaxation, which was abolished by tetrodotoxin and L-NA. The inhibitory effects of L-NA were antagonized by L-arginine (10?3 M). The presence of neurons containing nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase was histochemically demonstrated in the sphincter of Oddi. These findings may indicate that TES, nicotine and K+ liberate NO from NANC inhibitory nerve which is involved in the relaxation of the dog sphincter of Oddi. The muscular tone does not seem to be regulated by cholinergic nerves under the experimental conditions used. 相似文献
56.
ThedynamicdistributionofnitricoxidesynthaseinthesmallintestineofmicewithintestinalradiationsicknessWeiLichun(魏丽春);GuoYao(郭鹞)(... 相似文献
57.
Association of Vascular Endothelial Growth Factor Expression with Tumor Angiogenesis and with Early Relapse in Primary Breast Cancer 总被引:10,自引:0,他引:10
Masakazu Toi Seigo Hoshina Toshiaki Takayanagi Takeshi Tominaga 《Cancer science》1994,85(10):1045-1049
Angiogenesis is an independent prognostic indicator in breast cancer. In this report, the relationship between expression of vascular endothclial growth factor (VEGF; a selective mitogen for endothelial cells) and the microvessel density was examined in 103 primary breast cancers. The expression of VEGF was evaluated by immunocytochemical staining using anti-VEGF antibody. The microvessel density, which was determined by immunostaining for factor VIII antigen, in VEGF-rich tumors was clearly higher than that in VEGF-poor tumors ( P <0.01). There was a good correlation between VEGF expression and the increment of microvessel density. Furthermore, postoperative survey demonstrated that the relapse-free survival rate of VEGF-rich tumors was significantly worse than that of VEGF-poor tumors. It was suggested that the expression of VEGF is closely associated with the promotion of angiogenesis and with early relapse in primary breast cancer. 相似文献
58.
L- Tetrahydropalmatine (L- THP) ,a kind of al-kaloid extracted from traditional Chinese medicineRhizoma corydalis,posseses sedative,analgesic andhypnotic effects.Recently,it has been demonstratedthat L- THPalso had calcium- antagonistic and antiar-rythmic effects.Studies showed that L- THP had aprotective effect on acute focal and global cerebral is-chemia- reperfusion injury[1,2 ] .Many studies provedthat the production of NO wasincreased significantlyduring ischemia- reperfusion.The o… 相似文献
59.
We have sought to define a way in which nitrous oxide can be safely and universally used at minimal to low flows by utilising a circle system with a controlled leak provided by a standard gas analyser sampling line and a fresh gas supply of 50% nitrous oxide in oxygen, entering from a trunk interposed between the ventilator and the circle system. Although preliminary calculations suggested that this arrangement was likely to work, it was found that 13 of 23 patients studied prospectively developed an inspired oxygen fraction below 0.3. We conclude that, although this arrangement provides a new means of introducing nitrous oxide into the circle breathing system, it does not appear inherently safer or more convenient than the conventional route. 相似文献
60.
T.G. van Kooten J.M. Schakenraad H.C. van der Mei A. Dekker C.J. Kirkpatrick H.J. Busscher 《Medical engineering & physics》1994,16(6):506-512
In this study, human umbilical vein and human saphenous vein endothelial cells were seeded on glass and exposed to fluid shear in a parallel-plate flow chamber. Cell retention, morphology and migration were studied as a function of shear stress and of adhesion time prior to exposure to shear. Three-hour and 24-h adhesion times gave rise to comparable cell retention values after 2 h of flow for both cell types. Cell retention decreased from 85 to 20% as shear stress increased from 88 to 264 dynes cm−2 (8.8 to 26 Pa). Mean spreading areas decreased after the onset of flow, but subsequently stabilized to plateau values, which were smaller at higher shear stresses. Shape factors increased faster to higher values as cells were exposed to higher shear stresses, without any obvious preference in orientation of the cells with respect to the direction of flow. Migration was unidirectional with flow and linear with time. Migration was faster for cells which had adhered for 24 h than for cells which had adhered for 3 h and was accompanied by the presence of fibrillar structures left behind on the surface upstream of migrating cells. It is concluded that after 3 h adhesion to glass, cells have adhered with an adhesion strength that does not substantially increase during the next 21 h. However, during this time changes in cell-substratum interactions seem to occur judging by the differences in, e.g., migration rates. 相似文献